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Gibco™ Human EGF Recombinant Protein
Recombinant Protein
Marke: Gibco™ PHG0313
Beschreibung
Carrier-Free Reconstitution: Centrifuge the vial briefly before opening to bring the contents to the bottom. Reconstitute the lyophilized protein in sterile, PBS buffer to a concentration of 0.1-1.0 mg/mL. Make any further dilutions of the reconstituted protein in low endotoxin medium, or a buffered solution containing a carrier protein such as heat inactivated FCS or tissue culture grade BSA Storage: Store the lyophilized protein at 2°C to 8°C, preferably desiccated. Upon reconstitution, apportion into working aliquots and store at ≤-20°C. Avoid repeated freeze-thaw cycles.
EGF (Epidermal growth factor) exerts its actions by binding to the EGF Receptor, a 170 kDa protein kinase. Activation of EGFR initiates diverse cellular pathways in response to toxic environmental stimuli, or to EGF binding to the receptor, the EGFR forms homo- or heterodimers with other family members. Each dimeric receptor complex initiates a distinct signaling pathway by recruiting different Src homology 2 (SH2) containing effector proteins. EGF is far and wide expressed in kidney, cerebrum, prostrate and salivary glands. EGF acts as a potent mitogenic factor and the phosphorylated receptor recruits adapter proteins like GRB2 that activates complex downstream signaling cascades. EGF activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STAT modules. Research studies suggest the protein may also play important role in activating the NF-kappa-B signaling cascade. Defects in the EGGF gene are the cause of hypomagnesemia type 4 and dysregulation has been associated with the growth and progression of certain cancers.
Spezifikation
P01133 | |
protein with no preservative | |
6.2 kDa | |
Sequential Chromatography | |
<0.1 ng/ μg | |
EGF | |
Unconjugated | |
Recombinant | |
Carrier-Free | |
Wet Ice | |
Growth Factors & Receptors | |
Lyophilized | |
Stem Cell Research, Oncology | |
>95% by SDS-PAGE |
Control, ELISA, Functional Assay, Immunohistochemistry, Western Blot | |
1950 | |
Human EGF | |
1 mg | |
AI790464; beta-urogastrone; EGF; Epidermal growth factor; epidermal growth factor (beta-urogastrone); epidermal growth factor precursor; H-EGF; HOMG4; Pro-epidermal growth factor; Pro-epidermal growth factor precursor (EGF); sb:eu639; URG; Urogastrone | |
ED50 < 0.4 ng/mL; determined by the dose-dependent proliferation of mouse BALB/3T3 cells. | |
Human | |
4°C | |
<0.1 ng/μg | |
E. coli | |
Recombinant, Ligand | |
EGF (Epidermal Growth Factor) | |
Gibco™ |
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