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Thermo Scientific™ GeneBLAzer™ VDR DA Assay Kit

GeneBLAzer™ VDR DA (Division Arrested) cells and VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line.

Brand:  Thermo Scientific™ K1417

Product Code. 10461025

  • 1590.00 € / 1 Stück

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Nur für Forschungszwecke. Die Anwendung muss den Produktanweisungen entsprechen.

This item is not returnable. View return policy

Description

Description

GeneBLAzer™ VDR DA (Division Arrested) cells and VDR-UAS-bla HEK 293T cells contain the ligand-binding domain (LBD) of the human Vitamin D receptor (VDR) fused to the DNAbinding domain of GAL4 stably integrated in the GeneBLAzer™UAS-bla HEK 293T cell line. GeneBLAzer™ UAS-bla HEK 293T cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-VDR (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both VDR DA cells and VDR-UAS-bla HEK 293T cells are functionally validated for Z' and EC50 concentrations of Calcitriol (1α, 25-dihydroxyvitamin D3). In addition, VDR-UAS-bla HEK 293T cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time.

TRUSTED_SUSTAINABILITY
Specifications

Specifications

Zellbasiertes Beta-Lactamase-Reportergen
Versand: Trockeneisenlagerung: -80 °C/Flüssigstickstoff Ausreichend „Division Arrested“-Zellen und Substrat, für den Assay einer 384-Well-Platte. Lieferumfang: VDR-HEK-293T-DA-Zellen (K1417A)LiveBLAzer™-FRET B/G Ladekit, 70 μg
Kernrezeptoren, nukleäre Rezeptoren
VDR
TO901317
1 Platte
VDR, NR1I1
293T (HEK)
Kernrezeptoren
Zellen mit arretierter Teilung
Fluoreszent
7421
VDR HEK 293T DA Zellen (K1417A) LiveBLAzer™-FRET B/G Ladekit, 70 μg
GeneBLAzer™
BLA (Beta-Lactamase)
Zellen mit arretierter Teilung
GeneBLAzer™
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Zertifikate

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Chargennummer Art des Zertifikats Datum Catalog Number
Chargennummer885119 Art des ZertifikatsAnalysenzertifikat Datum15/04/2017 Catalog NumberK1417
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Thermo Scientific™ GeneBLAzer™ VDR DA Assay Kit > 1 Platte

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Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.