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IBA Lifesciences Strep-Tactin™ 4Flow™ high capacity 50% suspension
Strep-Tactin™ 4Flow™ high capacity 50% Suspension to purify Strep-tag™II or Twin-Strep-tag™ fusion proteins
Marke: IBA Lifesciences 2-1250-002
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Beschreibung
- Strep-Tactin™ 4Flow™ high capacity is a resin for purification of strep-tagged proteins via affinity chromatography
- Product is optimized for column-based use and suitable for HPLC/FPLC
- Recommended for all protein classes due to the broad pH compatibility, excellent pressure stability, outstanding target purity (>95%), and high binding affinity
- 4% crosslinked agarose beads coupled to Strep-Tactin™ for straightforward purification of proteins with Strep-tag™II or a Twin-Strep-tag™
- Targeted elution: no tedious titration steps required and multiple regeneration cycles possible
- Ideal alternative for proteins that are difficult to elute from Strep-Tactin™XT 4Flow™ resins
- Biotinylated proteins also bind to Strep-Tactin™ 4Flow™ high capacity and need to be blocked using BioLock
Spezifikation
| 20 mg/mL resin | |
| 50% suspension | |
| 50 to 150 μm | |
| 4 mL | |
| Proteins tagged with Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin | |
| Affinity chromatography equipment | |
| Agarose | |
| Bottle |
| 4 mL | |
| 4% agarose, crosslinked | |
| Resin Slurry | |
| Tetrameric protein | |
| Bulk Resin | |
| Twin-Strep-tag™; Strep-tag™II; cross reactivity: biotin | |
| Resin sufficient for 2 times 1 mL gravity flow column | |
| Suspension |