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R&D Systems™ Recombinant Human Wnt-3a Protein
Extensive quality control produces industry leading bioactivity and lot-to-lot consistency that instills confidence in results and ensures reproducibility. Applications: Bioactivity
Marke: R&D Systems™ 5036-WN-010/CF
304.96 EUR gültig bis 2024-12-16
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Beschreibung
Measured by its ability to induce alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 5.00-25.0 ng/mL. Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is <500 ng/mL. Protein concentrations should be titrated based on cell type and if appropriate, passage number of the cell line. Optimal concentrations should be determined by each laboratory for each application.Spezifikation
Lyophilized from a 0.2μm filtered solution in PBS, EDTA and CHAPS. | |
37.4 kDa | |
10 μg | |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70° C as supplied. 1 month, 2 to 8° C under sterile conditions after reconstitution. 3 months, -20 to -70° C under sterile conditions after reconstitution. | |
Recombinant |
89780 | |
Wnt-3a | |
Chinese Hamster Ovary cell line,CHO-derived human Wnt-3a protein Ser19-Lys352 | |
Unconjugated | |
75%, by SDS-PAGE with silver staining. |