missing translation for 'onlineSavingsMsg'
Learn More
Learn More
THP-1 (human acute monocytic leukemia) Whole cell lysate, Non-denatured, Abnova
Click to view available options
Menge:
200 μg
Packungsgröße:
200 Mikrogramm
Beschreibung
- Tissue: Peripheral blood
- Host: Human
- Lysis Buffer: Modified RIPA Lysis Buffer: 50mM Tris-HCl pH 7.4, 150mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
- Storage buffer: In modified RIPA Lysis Buffer
Western Blotting, Immunoprecipitation
Spezifikation
Spezifikation
| Zur Verwendung mit (Anwendung) | Immunoprecipitation, Western Blot |
| Tissue | Blood, peripheral |
| Beschreibung | Whole cell lysate (non-denatured) |
| Lysepuffer | Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF. |
| Vorbereitungsmethode | Cell lysate was prepared by homogenization in ice-cold modified RIPA Lysis Buffer with cocktail of protease inhibitors (Sigma). Cell debris was removed by centrifugation. Protein concentration was determined with Bradford assay (Bio-Rad protein assay, Microplate Standard assay). |
| Qualitätskontrollen | 12.5% SDS-PAGE Stained with Coomassie Blue. |
| Menge | 200 μg |
| Lagerungspuffer | In modified RIPA Lysis Buffer. |
| Wirtsspezies | Human |
| Konzentration | 5 mg/mL |
| Mehr anzeigen |
Berichtigung von Produktinhalten
Bitte geben Sie uns Ihr Feedback zu den Produktinhalten, indem Sie das folgende Formular ausfüllen.
Name des Produkts
Haben Sie Verbesserungsvorschläge?Übermitteln Sie eine inhaltliche Korrektur