TCR Vβ3 Hamster anti-Mouse, Brilliant Violet 510, Clone: KJ25, BD Optibuild™

Beschreibung

The KJ25 antibody specifically reacts with the V β 3 T-cell Receptor (TCR) of strains having the a (e.g., C57BR, SJL), b (e.g., AKR, CBA/Ca, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex. V β 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the Mtv-1 ( Mls-4[a], Mls[c] ), Mtv-3 ( Mls[c] ), Mtv-6 ( Mls-3[a], Mls[c] ), Mtv-13 ( Mls-2[a], Mls[c] ), Mtv-27, Mtv-44, and/or Mtv-MAI endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW). V β 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B. Activation or elimination of V β 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E. This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes.

The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes. With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set. BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser. BV510 is a polymer-based dye that is brighter than BD Horizon V500. Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.