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R&D Systems™ Recombinant Human Fc epsilon RI alpha Avi His Protein
Biotinylated Avi-tag His-tag
Marke: R&D Systems™ AVI6678-050
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Beschreibung
Measured by its binding ability in a functional ELISA. When Human IgE is immobilized at 1.00 μg/mL (100 μL/well), Biotinylated Recombinant Human Fc epsilon RI alpha Avi-tag His-tag binds with an ED50 of 0.300-1.80 ng/mL.Spezifikation
| P12319.1 | |
| Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. | |
| 50 μg | |
| Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20°C to -70°C as supplied. 1 month, 2°C to 8°C under sterile conditions after reconstitution. 3 months, -20°C to -70°C under sterile conditions after reconstitution. | |
| Biotin | |
| >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie™ Blue Staining. |
| Bioactivity | |
| MolecularWeight-observed: 50-60 kDa, under reducing conditions., MolecularWeight-theroretical: 25 kDa | |
| Chinese Hamster Ovary cell line, CHO-derived human Fc epsilon RI alpha protein Human FCER-1 alpha (Val26-Gln205) (N-terminus) Accession # P12319.1 Avi-tag 6-His tag (C-terminus) | |
| <0.10 EU / 1 μg of the protein by the LAL method. | |
| Reconstitute at 500 μg/mL in PBS. | |
| Fc epsilon RI alpha |