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O-GlcNAc Monoclonal Antibody (RL2), Alexa Fluor™ 647, eBioscience™, Invitrogen™
Mouse Monoclonal Antibody
Marke: Affymetrix eBioscience 51-9793-41
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Beschreibung
Description: This RL2 monoclonal antibody recognizes proteins with O-N-acetylglucosamine (O-GlcNAc) glycosylation. It was originally developed by immunizing mice with the rat liver nuclear envelopes containing nuclear pore complexes. The RL2 clone has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and flow cytometry in a wide variety of mammalian cells. Applications Reported: This RL2 antibody has been reported for use in intracellular staining followed by flow cytometric analysis. Applications Tested: This RL2 antibody has been pre-diluted and tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to Best Protocols: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This may be used at 5 μL (0.5 μg) per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser
O-GlcNAc Monoclonal antibody specifically detects O-GlcNAc in All, Human samples. It is validated for Flow Cytometry
Spezifikation
| O-GlcNAc | |
| Monoclonal | |
| 5 μL/Test | |
| PBS with 0.1% gelatin, 0.2% BSA and 0.09% sodium azide; pH 7.2 | |
| O15294 | |
| OGT | |
| Rat liver nuclear envelopes | |
| 25 Tests | |
| Primary | |
| All species, Human | |
| Antibody | |
| IgG1 κ |
| Flow Cytometry | |
| RL2 | |
| Alexa Fluor 647 | |
| OGT | |
| 1110038P24Rik; 4831420N21Rik; AI115525; GlcNAc; HINCUT-1; HRNT1; O-GLCNAC; O-GlcNAc transferase subunit p110; Ogt; Ogtl; O-linked N-acetylglucosamine transferase 110 kDa subunit; UDP-N-acetylglucosamine--peptide N-acetylglucosaminyltransferase 110 kDa subunit | |
| Mouse | |
| Affinity chromatography | |
| RUO | |
| 8473 | |
| 4° C, store in dark, DO NOT FREEZE! | |
| Liquid |
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