Invitrogen™ HLA-BC Monoclonal Antibody (B1.23.2), Functional Grade, eBioscience™, Invitrogen™

Beskrivelse

Description: This B1.23.2 monoclonal antibody recognizes the human Human Leukocyte Antigens (HLA) B and C. Along with HLA-A, these proteins correspond to MHC Class I in humans. HLA-B and HLA-C are expressed on nearly all cell types and heterodimerize with beta-2 microglobulin at the cell surface. These molecular complexes play a critical role in the presentation of endoplasmic reticulum-derived peptides to immune cells. The antibody has been shown to inhibit binding of KIR2DL1 and LIR1. Applications Reported: This B1.23.2 antibody has been reported for use in flow cytometric analysis. Applications Tested: This B1.23.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.5 μg per test. A test is defined as the amount (μg) of antibody that will stain a cell sample in a final volume of 100 μL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Filtration: 0.2 μm post-manufacturing filtered. Purity: Greater than 90%, as determined by SDS-PAGE. Endotoxin Level: Less than 0.001 ng/μg antibody, as determined by LAL assay. Aggregation: Less than 10%, as determined by HPLC.

HLA-class I antigens are widely distributed on human nucleated cells. The intensity of HLA-ABC maybe altered in pathological states e.g. malignant cells may lose HLA-ABC, whereas hepatocytes in alcoholic hepatitis, biliary cirrhosis and chronic active hepatitis may show enhanced expression of HLA-ABC. HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.