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Thermo Scientific™ GeneBLAzer™ ERα-UAS-bla GripTite™-Zellen
ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line.
Marke: Thermo Scientific™ K1688
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Beschreibung
ER alpha-UAS-bla GripTite™cells contain the ligand-binding domain (LBD) of the human Estrogen receptor alpha (ER alpha) fused to the DNA-binding domain of GAL4 stably integrated in the GeneBLAzer™ UAS-bla GripTite™ cell line. GeneBLAzer™ UAS-bla GripTite™ cells stably express a beta-lactamase reporter gene under the transcriptional control of an upstream activator sequence (UAS). When an agonist binds to the LBD of the GAL4 (DBD)-ER alpha (LBD) fusion protein, the protein binds to the UAS, resulting in expression of beta-lactamase. Division Arrested (DA) cells are available in two configurations- an Assay Kit (which includes cells and sufficient substrate to analyze 1 x 384-well plate), and a tube of cells sufficient to analyze 10 x 384-well plates. ER alpha-UAS-bla GripTite™ cells are functionally validated for Z' and EC50 concentrations of 17-beta Estradiol. In addition, ER alpha-UAS-bla GripTite™ cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time (data available upon request). Additional testing data using alternate stimuli are also available.
Spezifikation
| Betamethason, Cortisol, Budesonid | |
| Zellbasiertes Beta-Lactamase-Reportergen | |
| Flüssiger Stickstoff, Raumtemperatur | |
| GeneBLAzer™ ERα-UAS-bla GripTite™ Zellen, 2 x 106 Zellen, ∽2,000,000 Zellen/Fläschchen bei 2,000,000 Zellen/ml Zellmenge, Cortison-/Mifepriston-/Progesteron-Antagonisten, Fluoreszenznachweisverfahren, GPCRs Druggable Target, BLA (Beta-Lactamase)-Reporter, Lagerung in Flüssigstickstoff | |
| Human | |
| ER alpha-UAS-bla GriptTite™ Zellen | |
| Dexamethason | |
| 2 x 10^6 Zellen | |
| GripTite™ Zellen | |
| Raumtemperatur | |
| GeneBLAzer™ ERα±-UAS-bla GripTite™ Zellen |
| Milfepristone, Cortison, Progesteron | |
| Teilende Zellen | |
| Fluoreszent | |
| Pharmazie und Biopharmazie, Arzneimittelforschung und -entwicklung, Ziel- und Verlaufserkennung und -validierung, G-Protein-gekoppelte Rezeptorbiologie, zellbasierte GPCR-Reporter-Assays | |
| Kernrezeptoren, nukleäre Rezeptoren | |
| 2 x 106 Cells | |
| GeneBLAzer™ | |
| GripTite Zellen | |
| BLA (Beta-Lactamase) | |
| ER Alpha, ESR1, ERA, NR3A1 |
Nur für Forschungszwecke. Nicht zur Verwendung bei diagnostischen Verfahren.