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Thermo Scientific™ Fluorescein-D22 Coactivator Peptide, 100 μM
LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays.
Marke: Thermo Scientific™ PV4387
Weitere Details : Netto-Gewicht : 0.50000kg
Beschreibung
LanthaScreen™ fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen™ TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.
How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen™ panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen™ TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).
Spezifikation
| ≥ 95% Reinheit, bestimmt durch HPLC | |
| 100μ m | |
| Fluorescein-D22Koaktivator-Peptid, 1 ml, Konzentration 100 μM, Molekulargewicht 2499 Da, Lagerungsbedingung -5 bis -30 °C, für Pharma und Biopharma, Target- und Bleiidentifizierung und -validierung | |
| Klassische Farbstoffe | |
| Fluorescein – D22 Koaktivator-Peptid | |
| Nur für Forschungszwecke Darf nicht für diagnostische Verfahren eingesetzt werden. | |
| Trockeneis |
| Fluorescein – D22 Koaktivator-Peptid | |
| Lagern Sie die Peptide bei -20 °C. | |
| Pharma und Biopharma, Ziel- und Lead-Erkennung und -Validierung, Arzneimittelforschung und -entwicklung, Biologie der Kernrezeptoren, Kernrezeptorproteine, biochemische Kernrezeptor-Assays, Kernrezeptor-Koregulatorinteraktion | |
| 2499 Da | |
| 1 ml | |
| Fluorescein- LPYEGSLLLKLRAPVEEV | |
| 10 Mm Na2CO3 (pH 11.0) |
For Research Use Only. Not for use in diagnostic procedures.