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Invitrogen™ Dynabeads™ RNA Purification Kit
The Dynabeads RNA Purification Kit contains Dynabeads RNA Purification Buffer and Dynabeads Carboxylic Acid for RNA Purification, providing a simple, flexible, and automation-ready solution for purification of crude RNA produced by in vitro transcription (IVT).
Marke: Invitrogen™ 65032D
702.40 EUR gültig bis 2024-11-30
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Beschreibung
The Dynabeads RNA Purification Kit contains Dynabeads RNA Purification Buffer and Dynabeads Carboxylic Acid for RNA Purification beads, providing a simple, flexible, and automation-ready solution for purification of crude RNA produced by in vitro transcription (IVT). It is particularly suitable where high purity and scalability are required, such as in the discovery phase of RNA-based vaccine and therapeutic development.
Features of the Dynabeads RNA Purification Kit include:
• Efficiency—reuse at least six times to help reduce the cost and time in a simple workflow
• Scalability—swap between manual and automated workflows to match required scale (micrograms to grams of mRNA) and throughput
• Quality—ensure a safe and flexible supply of products to navigate from RNA-based drug discovery research to commercial manufacturing with counterpart solutions (available via the OEM commercial supply), without incurring extra time or costs from switching technology or raw material
Overview of the workflow
Crude RNA from the IVT step is mixed with the Dynabeads Carboxylic Acid for RNA Purification and Dynabeads RNA Binding Buffer is added. The RNA binds to the bead surface and the remaining components of the reaction mixture are removed by applying a magnet (such as thermofisher.com/magnets) and discarding the supernatant (Figure 1).
The products offered in this combo kit can also be purchased separately. In addition, they are included in the Dynabeads In Vitro Transcription and RNA Purification combo kit (Cat. No. 65034D) together with Dynabeads Streptavidin for In Vitro Transcription.
Automation-ready for use with KingFisher purification systems
The 1-μm magnetic beads are ideal for high-throughput screening. The process can be automated using liquid handlers or any of our KingFisher sample purification systems. The scripts for KingFisher Flex and Apex automated RNA purification workflows are available for download from our website.
Commercial supply
To learn more about counterpart products and support documentation available for GMP-compliant manufacturing of mRNA vaccines and therapeutics, visit thermofisher.com/dynabeadsmrna
Spezifikation
| • 1 unit of Dynabeads Carboxylic Acid for RNA Purification (Cat. No. 65021D). Store at 2–8°C. • 1 unit of Dynabeads RNA Binding Buffer (Cat. No. 65042D). Store at 2–8°C. Protect from light exposure during storage. |
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| Magnetic Bead | |
| Crude RNA | |
| KingFisher™ Sample Purification System, DynaMag™ magnets | |
| Dynabeads™ | |
| Umgebungstemperatur |
| Large Combo Kit | |
| Variable | |
| Ja | |
| In Vitro Transcription, Synthetic RNA, Crude RNA Purification (from IVT Reaction Mix) | |
| RNase-free (No detectable activity level) | |
| Unsteril |