Collagen I Polyclonal Antibody, Invitrogen™

Beschreibung

This product requires antigen retrieval using 1 mg/mL pepsin in 0.5M acetic acid for 2 hours at 37oC and multiple buffer washes prior to staining on paraffin sections. Reconstitute with 0.5 mL of distilled water. Prior to reconstitution, store at 4oC. After reconstitution, store undiluted at -20oC, avoiding freeze/thaw cycles. For long term storage the addition of 1% BSA and 0.09% sodium azide is recommended. Rabbit anti Human collagen I antibody recognizes human Collagen I, reacting with both native and heat denatured proteins, and has been cross-absorbed against Collagen types III, IV and V.

Collagen I is a Type I collagen with a triple helix structure comprised of two alpha-1 chains and one alpha-2 chain. Collagen I is a member of group I collagen (fibril-forming collagen) found in most connective tissues, and is abundant in bone, cornea, dermis and tendon. Mutations in the COL1A2 gene encoding the alpha-2 chain are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIB, recessive Ehlers-Danlos syndrome Classical type, idiopathic osteoporosis, and atypical Marfan syndrome. Symptoms associated with mutations in the COL1A2 gene which codes for the alpha-2 chain, however, tend to be less severe than mutations in the COL1A1 gene, reflecting the different role of alpha-2 chains in matrix integrity. Reciprocal translocations between chromosomes 17 and 22, where Collagen Type 1 genes and the gene for platelet derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified.