CCL5 (RANTES) Polyclonal Antibody, Biotin, PeproTech®, Invitrogen™

Beschreibung

AA Sequence of recombinant protein: SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human RANTES (CCL5). Anti-Human RANTES (CCL5)-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect hRANTES by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25-1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human RANTES (500-P36) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant hRANTES. Western Blot: To detect hRANTES by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant hRANTES is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P36BT-1MG will be provided as 2 x 500 μg

CCL5 (RANTES) is a chemokine gene located on the q-arm of chromosome 17. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. CCL5, a member of the CC subfamily, functions as a chemoattractant for blood monocytes, memory T helper cells, and eosinophils. It causes the release of histamine from basophils and activates eosinophils. This cytokine is one of the major HIV-suppressive factors produced by CD8+ cells. It functions as one of the natural ligands for the chemokine receptor chemokine (C-C motif) receptor 5 (CCR5), and it suppresses in vitro replication of the R5 strains of HIV-1, which use CCR5 as a coreceptor. Alternative splicing results in multiple transcript variants that encode different isoforms. Diseases associated with CCL5 include Human Immunodeficiency Virus Type 1 and Periapical Granuloma.