BD Biosciences™ Anti-Human IFN-γ FITC/CD69 PE/CD4 PerCP-Cy™5.5/CD3 APC (RUO/GMP)

Beschreibung

• Clone SK7 (also known as Leu-4) 25723.11 L78 SK3 (also known as Leu3a) (Ruo (GMP)).
• Anti-Hu–IFN-γ, clone 25723.11, is derived from the hybridization of mouse P3X-63- Ag8.653 myeloma cells with lymph node cells from BALB/c mice immunized with recombinant human IFN-γ.
• CD69, clone L78, is derived from hybridization of mouse Sp2/0-Ag14 myeloma cells with lymph node cells from BALB/c mice immunized with a CD8⁺ alloantigen-directed cytotoxic T-lymphocyte (CTL) cell line.
• CD4, clone SK3, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immununized with human peripheral blood T lymphocytes.
• CD3, clone SK7, is derived from hybridization of mouse NS-1 myeloma cells with spleen cells from BALB/c mice immunized with human thymocytes.
• Anti-Human Interferon- γ (Anti-Hu–IFN-γ) recognizes a 20- to 25-kilodalton (kd) glycoprotein.
• CD69 recognizes a very early human activation antigen. The CD69 antigen is a surface homodimer formed by the association of 28- and 32-kd chains that are held together by disulfide bridges.
• The CD4 antigen, Mr 55 kd, is present on T-helper/inducer lymphocytes and monocytes.
• CD3 reacts with the epsilon chain of the CD3 antigen/T-cell antigen receptor (TCR) complex. This complex is composed of at least six proteins that range in molecular weight from 20 to 30 kd.
• The antigen recognized by CD3 antibodies is noncovalently associated with either α/β or γ/δ TCR (70 to 90 kd).
• Contents:
• Specificity: CD3, Clone: SK7 (also known as Leu4), Format: APC, Isotype: IgG₁K, Entrez Gen ID: 916
• Specificity: IFN-γ, Clone: 25723.11, Format: FITC,Isotype: IgG_2bK, Entrez Gen ID: 3458
• Specificity: CD69, Clone: L78, Format: PE, Isotype: IgG₁K, Entrez Gen ID: 969
• Specificity: CD4, Clone: SK3 (also known as Leu-3a), Format: PerCP-CY™5.5, Isotype: IgG₁K, Entrez Gen ID: 920
• Research applications include the following:
  • Studies of T-cell responses to antigens such as cytomegalovirus (CMV), human immunodeficiency virus (HIV), herpes viruses, and tumor antigens.