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BTX™ AgilePulse™ MAX Large Volume Transfection System
AgilePulse™ MAX Large Volume Transfection System provides an advanced electroporation solution for fast, efficient transfection of up to 10 mL of cell suspension.
Marke: BTX™ 470200N
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Beschreibung
Includes a user-friendly, programmable waveform generator with patented AgilePulse™ technology, BTX™ patent-pending large-volume electroporation chamber, and proprietary BTXPress™ Cytoporation Medium T, optimized for large volume electroporation. IQ/OQ Now available upon request.
- Scale-up
- Transfection protocols readily scale-up from standard laboratory cuvettes to large-volume transfection in the AgilePulse™ MAX system.
- Maximal Efficiency with Cytoporation Medium
- BTXPress™ Cytoporation Medium T used with the AgilePulse™ MAX system has been optimized for maximal efficiency with a number of cell lines, including K562, A20, HEK293 and CHO-KI.
- Compatible with a large range of transfectants including DNA, RNA, siRNA, and olignonucleotides.
- Can be directly diluted in complete growth medium for post-electroporation cell culture.
- Simple User Interface
- All controls are operated with the simple touch screen on the front panel.
- Data is quickly retrieved by USB key and can be analyzed for detailed pulse characteristics including pulse and pulse current.
- AgilePulse™ Advantage
- Transfection efficiency and cell viability are enhanced by specialized, programmable electrical pulse waveforms, particularly important for larger polynucleotide delivery such as DNA plasmids.
- The patented AgilePulse™ technology combines a unique sequence of short high-intensity pulses to porate cell membranes, followed by long low-intensity pulses to further drive transfectants into cells via electrophoresis, while maintaining cell viability.
Spezifikation
Large volume | |
BTXpress Cytoporation Medium T oder Zytoporation Medium T4, Elektroporationskammer mit großem Volumen | |
BTX-Elektroporation | |
50 bis 1000 V | |
Touchscreen | |
40 cm | |
20 cm | |
50 bis 104 °F | |
10°bis 40 °C | |
Je 1 | |
25 lb. | |
12,6 in. | |
Unsteril | |
Touchscreen-Display, Fußschalter |
CRISPR-Transfektionen, Transfektion von Zellen wie Knochenmark zur Produktion oder zum Ersatz eines fehlenden Proteins, Abgabe von siRNA zur Unterdrückung der Genexpression, Abgabe von Genen für die permanente Genkorrektur, Beladen von Zellen mit einem Medikament zur Medikamentenverabreichung, Krebsimmuntherapie, Transfektion eukaryotischer Zellen zur Proteinproduktion in Bioreaktoren, großmaßstäbliche Produktion von replikationsdefizienten Viren | |
5 kHz bis 1 Hz | |
Complete Electroporation System | |
AgilePulse MAX Large Volume Transfection System, Complete | |
15,7 in. | |
7,9 in. | |
AgilePulse™ MAX Generator, Large Volume Chamber Stand, Safety Stand, 5 mL Chamber (2 X), Cytoporation Medium T (500 mL) and 4 mm gap Cuvettes | |
0,200 bis 1000 ms | |
0,050 bis 10 ms | |
Bis zu 10 ml | |
11.3 kg | |
32 cm | |
Kammerkabel | |
Rechteckwelle |